DOT Drug Tests Get 'Biological Truth' Overhaul: Urine Wins Again
Published Date: 10/1/2025
Proposed Rule
Summary
The Department of Transportation is updating its drug and alcohol testing rules to match a new executive order focused on protecting biological truth. This means some testing terms will change, and in certain cases, workers will need to provide urine samples instead of oral fluid tests, which aren’t ready yet. These updates mainly affect transportation workers and companies, with no big cost changes announced yet.
Analyzed Economic Effects
1 provisions identified: 0 benefits, 1 costs, 0 mixed.
Urine Tests Replace Oral Fluid
If you are a transportation worker, the Department of Transportation proposes that in some situations you must provide a directly observed urine sample instead of an oral fluid test because oral fluid testing is not yet available. This supplement aligns testing terms with Executive Order 14168 and was published after an earlier NPRM on December 9, 2024.
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